Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes

Figure 1 Different morphology and differentiation of HUCB-derived MSCs. A: morphology of primary cultured HUCB-derived MSCs. MSCs are spindle-like and osteroclasts are big and round; B: morphology of sub-cultured HUCB-derived MSCs stained with HE. The fibroblast-like cells are growing as a whirlpool ( ×100); C: representative immuocytochemistry showing expression of CK-18 on HUCB-derived MSCs on d 28 (SABC×200); D: cells stained by PAS. Glycogen storage is seen as accumulated magenta staining, the round and epithelioid cells show magenta staining in the region (×400).