Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis

Figure 1 PCR profiles of total DNA recovered from human blood samples and of E coli DNA (control) obtained using primers BD1 and BD2 specific for bacterial 16S rRNA region. Positive control: Chromosomal DNA from E coli. Lanes 1-11: total DNA extracted from human blood samples.

Figure 2 PCR profile of E coli chromosomal DNA. Lanes 1-3: DNA amplified using primers BG1 and BG4 specific for E coli. Lanes 4-6: DNA amplified using primers BFR1 and BFR2 specific for Bacteroides spp. Lane 7: 1 kb standard DNA ladder.

Figure 3 DGGE profiles of the amplified region of bacterial DNA coding for 16S rRNA using primers F3 and Rev 2. Positive controls: Lane 3. Staphylococcus aureus; Lane 4. Pseudomonas aeruginosa; Lane 5. Bacillus cereus; Lane 6. E coli; Lanes 1-2 and 7-13: bacterial DNA present in human blood samples.