Enteroaggregative Escherichia coli isolated from Chinese diarrhea patients with high-pathogenicity island ofYersinia is involved in synthesis of siderophore yersiniabactin

Figure 1 PCR amplification results of 12 genes within HPI core part. Lane 1: fyuA, lane 2: irp5, lane 3: irp4, lane 4: irp3, lane 5: irp1, lane 6: irp2, lane 7: ybtA, lane 8: irp6, lane 9: irp7, lane 10: irp8, lane 11: irp9, lane 12: asnT-tRNA-int, M: DL2000 marker.

Figure 2 Fluorescence of fyuA-gfp in E. coli grown on LB media (green curve) and LBD media (black curve). A: WA-CS irp1::KN(pCJG3.3N), CFT073(HPI+), HB101(HPI–) and EAEC 47/37(HPI–); B: WA(HPI+); C: EAEC 66(HPI+); D: EAEC SS23(HPI+); E: EAEC B86-2(HPI+); F: EAEC E1073(HPI+); G: EAEC 52/46(HPI+); H: EAEC 17-2 (HPI+); I: EAEC O42 (HPI+); M1: specified range of background of fluorescence signal; M2: specified range of enhanced florescence signal.