Screening of genes of proteins interacting with p7 protein of hepatitis C virus from human liver cDNA library by yeast two-hybrid system

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Aug 14, 2005 (publication date) through Apr 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Aug 14, 2005; 11(30): 4709-4714
Published online Aug 14, 2005. doi: 10.3748/wjg.v11.i30.4709

Figure 1 Map of “bait” plasmid pGBKT7-p7.

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Figure 2 Fragment-p7 amplified by PCR (A) and pGBKT7-p7 cut by EcoRI/ BamHI (B).

Figure 3 Expression of HCV p7 in yeast shown by Western blotting. Lane 1: HCV p7 protein; lane 2: positive control; lane 3: negative control.

Figure 4 Identification of different colonies with BglII digestion.

Citation: Huang YP, Zhang SL, Cheng J, Wang L, Guo J, Liu Y, Yang Y, Zhang LY, Bai GQ, Gao XS, Ji D, Lin SM, Zhong YW, Shao Q. Screening of genes of proteins interacting with p7 protein of hepatitis C virus from human liver cDNA library by yeast two-hybrid system. World J Gastroenterol 2005; 11(30): 4709-4714
URL: https://www.wjgnet.com/1007-9327/full/v11/i30/4709.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i30.4709