Viral Hepatitis
Copyright ©The Author(s) 2005.
World J Gastroenterol. Jul 7, 2005; 11(25): 3899-3904
Published online Jul 7, 2005. doi: 10.3748/wjg.v11.i25.3899
Figure 1
Figure 1 Structure of “bait” plasmid pGBKT7-complete S.
Figure 2
Figure 2 One thousand three hundred and thirty eight basepair fragment-complete S amplified by RT-PCR (A), pGBKT7-complete S cut by EcoRI/ BamHI (B), a 945 bp fragment-CSBP1 amplified by RT-PCR (C), pGBKT7-CSBP1 and pGADT7-CSBP1 cut by EcoRI/BamHI (D).
Figure 3
Figure 3 Expression of HBV complete S and HBV CSBP1 protein in yeast confirmed by Western blotting. Lane 1: HBV complete S protein; lane 2: positive control; lane 3: HBV CSBP1 protein.
Figure 4
Figure 4 Identification of different colonies with BglII digestion.
Figure 5
Figure 5 Interaction between HBV complete S protein and CSBP1 protein identified by coimmunoprecipitation. Lane 1: HBV complete S protein; lane 2: interaction with two proteins.