Incidence of HBV variants with a mutation at nt551 among hepatitis B patients in Nanjing and its neighbourhood

Figure 1 Amplification results of msPCR. A: Amplification of control HBV DNA. Lane 1: DNA marker; lane 2: HBV DNA with an A at nt551; lane 3: HBV DNA with G at nt551; lane 4: HBV DNA with C at nt551; lane 5: HBV DNA with T at nt551. B: The msPCR amplification for No.127, No.210 and No.216 by using P551G-PPS. Lane 1: DNA marker; lanes 2-6: No.8 of nt551A (negative control), No.57 (positive control), No.127, No.210 and No.216 were amplified by P551G-PPS respectively. C: The msPCR amplification for No.127, No.210 and No.216 by using P551C-PPS. Lane 1: DNA marker; lanes 2-6: No.8 of nt551A (negative control), HBV DNA of nt551C (positive control), No.127, No.210 and No.216 were amplified by P551C-PPS respectively. D: The msPCR amplification for No.127, No.210 and No.216 by using P551T-PPS. Lane 1: DNA marker; lanes 2-6: No.8 of nt551A (negative control), No.2 of nt551T(positive control), No.127, No.210 and No.216 were amplified by P551T-PPS respectively.