Generation of the regulatory protein rtTA transgenic mice

Figure 1 Schematic illustration of the ApoE-rtTA transgenic construct used to generate ApoE-rtTA transgenic mice.

Figure 2 Identification of pApoE-rtTA. Lane M3: DL2 000 + DL15 000 (TaKaRa); Lane 1: pLiv.7 cut by KpnI and HpaI; Lane 2: purified rtTA fragment; Lane 3: pApoE-rtTA cut by KpnI and HpaI; Lane 4: pApoE-rtTA digested by NotI and SpeI.

Figure 3 Mouse genotyping for the potential ApoE-rtTA transgenic founders (A and B) and subsequent generation(s) (C). A: Diagnostic PCR results for the presence and absence of ApoE-rtTA gene from genomic DNA of the potential transgenic founders; B: Southern blot analysis for detection of integrated ApoE-rtTA transgene within founder mouse genome; C: Genotyping for the subsequent generation (F1) derived from transgenic founder C1.

Figure 4 mRNA expression in ApoE-rtTA transgenic mice. A: RT-PCR analysis was performed to examine expression of rtTA in transgenic mouse liver; B: Tissue-specificity of rtTA transgene expression in transgenic animals.