Presence and integration of HBV DNA in mouse oocytes

Figure 1 Electrophoresis of amplification products of DNA from mouse oocytes. Lane 1: markers; lane 2: negative control; lane 11: positive control; lanes 3-10: amplification products of DNA from group A oocytes in eight experiments.

Figure 2 Southern blot results of amplification products of DNA from mouse oocytes. Lane 1: Markers; lane 2: negative control; lane 11: positive control; lanes 3-10: amplification products of DNA from group A oocytes in eight experiments.

Figure 3 Dot hybridization for the washing solution. Dots (1,1): negative control; Dots (4,6): positive control; Dots (1,2) to (1,7), (2,1) to (2,7), (3,1) to (3,7), (4,1) to (4,4): the washing solution samples from group A in eight experiments.

Figure 4 FISH results. A: No signal of HBV DNA integration in the metaphase from group B oocytes. B-D: Clear signals of HBV DNA integration in the metaphases from group A oocytes (arrow).