Colorectal Cancer
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Apr 21, 2005; 11(15): 2255-2259
Published online Apr 21, 2005. doi: 10.3748/wjg.v11.i15.2255
Figure 1
Figure 1 Inhibition of proliferation of HCT cells by Verapamil. Results were expressed as mean±SD n = 9 bP<0.01 vs control.
Figure 2
Figure 2 The percentage of S phase HCT cells treated with Verapamil. With increase in Verapamil concentration, the percent of S phase cells was decreased as detected by flow cytometry.
Figure 3
Figure 3 Flow cytometry detection of HCT cells apoptosis. The percentage of apoptotic cells increased as Verapamil concentration increased. A: control; B: 1 μmol/L Verapamil; C: 10 μmol/L Verapamil; D: 100 μmol/L Verapamil; E: 500 μmol/L Verapamil.
Figure 4
Figure 4 DNA Ladder of HCT cell line treated with Verapamil. M: marker (100 bp DNA Ladder); Con: control; Vp1: 100 μmol/L Verapamil; Vp2: 200 μmol/L Verapamil; Vp3: 400 μmol/L Verapamil.
Figure 5
Figure 5 Cellular morphologic changes (400×). Cell membrane blebs and the cytoplasm and nuclear chromatin condensation in HCT cells treated with Verapamil were observed. A: control cells; B: 100 μmol/L Verapamil.
Figure 6
Figure 6 Nuclear model change as reniform and nuclear chromatin condensation in HCT cell line treated with Verapamil and labeled with PI (400×). A: Control cells; B: 100 μmol/L Verapamil.
Figure 7
Figure 7 Influence of Verapamil on [Ca2+]i intensity in HCT cell after 12 h (400×). 100 μmol/L. A: Control cells; B: 100 μmol/L Verapamil.