Expression and significance of nuclear factor κB p65 in colon tissues of rats with TNBS-induced colitis

Figure 1 HE staining showed inflammatory infiltration of colon tissue in model group×200. A lot of inflammatory cells infiltrated into the colon tissue.

Figure 2 levels of NF-κB p65 in colon tissue of rats with TNBS-indued colitis.

Figure 3 Western blotting showed levels of NF-κB P65 in colon tissue of rats. A: Rats of model group II exhibited higher levels of nuclear NF-κB P65 than others; B: total levels of NF-κB P65 were not different in rats. Lanes 1–4: model II, model I, NS, normal groups.

Figure 4 Immunohistochemical staining for TNF-α protein expression. SP×200. A: TNF-α protein expression in normal group; B: TNF-α expression in model II group. The number of TNF-α immunoreactive positive cells in model group was significantly more than that of normal controls.

Figure 5 Immunohistochemical staining for ICAM-1 protein expression. SP×200. A: Expression of ICAM-1 in normal group; B: Expression of ICAM-1 in model II group. The ICAM-1 expression was upregulated markedly in model group.