Hypoxia-inducible factor 1 alpha and vascular endothelial growth factor overexpression in ischemic colitis

Figure 1 RT-PCR analysis of HIF-1 alpha (lane 1: negative control; lane 2: rectal mucosa) and VEGF (lane 3: negative control; lane 4: rectal mucosa).

Figure 2 HIF-1 alpha RNA expression levels in the colon tissues.

Figure 3 VEGF RNA expression levels in the colon tissues.

Figure 4 HIF-1 alpha and VEGF RNA expression levels in the colon tissues in the active and healing phases of ischemic colitis.

Figure 5 Immunostaining of HIF-1 alpha or VEGF in the colon tissues. Weak HIF-1 alpha or VEGF immunoreactive cells were scattered in epithelial cells and interstitial cells in normal colon tissue (A: HIF-1 alpha; B: VEGF). In contrast, strong HIF-1 alpha or VEGF immunoreactive cells were diffusely seen in the epithelial and intestinal cells, including inflammatory cells in the ischemic colitis lesions (C: HIF-1 alpha; D: VEGF). Scale bars represent 100 μm.