H Pylori
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jan 7, 2005; 11(1): 114-117
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.114
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 Agarose gel electrophoresis analysis of recombinant pIRES-hpaA. Lane 1: PCR product of pIRES as a negative control; lane 2: PCR product of pIRES-hpaA; lane 3: pIRES-hpaA after digestion with EcoR I and Mlu I; lane 4: pIRES after digestion with Eco RI and Mlu I; lane 5: DNA Marker (DL2000+15000).
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Agarose gel electrophoresis analysis of recombinant attenuated Salmonella typhimurium DNA vaccine strain with restriction enzyme digestion. Lane 1: DNA ladder (1 kb); lanes 2-5: Recombinant plasmid pIRES-hpaA from strains of different generations after digestion with EcoRI and Mlu I; lane 6: pIRES after digestion with EcoRI and Mlu I.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Identification of recombinant attenuated Salmonella typhimurium DNA vaccine strain carrying hpaA by PCR. Lane 1: Product amplified from pIRES as a negative control; lane 2: Marker (DL2000+15000); lanes 3-4: Products amplified from recombinant plasmid from strains of different generations by PCR.
Figure 4
Open in New Tab Full Size Figure Download Figure
Figure 4 Western blotting of expressed pIRES-hpaA products. Lane 1: COS-7 cells transfected by pIRES as a control; Lane 2: COS-7 cells transfected by pIRES-hpaA.