Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

Figure 1 Representatives of esophageal cancer deregulated genes expressed in C57BL/6J mouse embryogenesis by re-verse northern screening. Three filters were hybridized with ³²P-labeled cDNA probe from E11.5, E13.5 and P1 period. β-actin and GAPDH were used as controls. c1, negative control; c2, β-actin; c3, GAPDH; 1, S100A8; 2, IGL; 3, RPL15; 4, CSTB; 5, SPRR2A; 6, RHCG; 7, keratin 4; 8, EMP1; 9, S100A9.

Figure 2 Expression patterns of several genes confirmed by multiplex semi-quantitative RT-PCR. All RT-PCR products were analyzed by electrophoresis on 15 g/L agarose gel. The ex-pression of GAPDH was used as internal control and the size of GAPDH gene amplification was 452 bp.