Helicobacter species sequences in liver samples from patients with and without hepatocellular carcinoma

Figure 1 Agarose gel (2%) showing PCR products obtained with Helicobacter genus-specific 16S ribosomal RNA primers. Lane 1 negative control (DEPC-treated water). Lanes 3 and 4 liver samples from patients with metastatic liver cancer. Lanes 2, 5, 6, 7, 8 and 9 positive liver samples. Lane 10 positive control (H. hepaticus DNA). MW molecular-weight marker (VCS Hae III).

Figure 2 Analysis of cagA-specific PCR products from Helicobacter genus positive liver samples of patients with cirrhosis and HCC. The 290 bp fragments were examined on 2% agarose gel electrophoresis. Lane 1 negative control (DEPC-treated water). Lanes 2 to 6 Helicobacter genus positive liver samples. Lanes 7 and 8 positive controls (cagA-cDNA). MW molecular-weight marker (VCS Hinf I). Lanes 2, 3, 5, and 6 positive for amplified cagA fragment. Lane 4 negative for cagA.