Antiangiogenic effect of somatostatin receptor subtype 2 on pancreatic cancer cell line: Inhibition of vascular endothelial growth factor and matrix metalloproteinase-2 expression in vitro

Figure 1 Immunohistochemical staining of SSTR2 expression after stable transfection The cytoplasmic brown yellow staining represents SSTR2 expression. (A) experimental group, (B) vector control, and (C) mock control (SABC × 150).

Figure 2 Expression of SSTR2 mRNA by RT-PCR analysis in experimental group, but not in vector control and mock control. Lane M: DNA marker DL 2000, Lane 1: experimental group, Lane 2: vector control, Lane 3: mock control.

Figure 3 Inhibition of VEGF production in cultured superna-tants by SSTR2. VEGF was determined in cultured superna-tants by ELISA in the first week and two months after stable transfection. VEGF secretion was significantly reduced in the experimental group compared with the vector and mock con-trols (P < 0.05). Values were expressed as mean ± SD.

Figure 4 Weak expression of VEGF mRNA in experimental group but strong expression in vector and mock control shown by RT-PCR analysis. The expected length of PCR products was 240 bp (VEGF) and 520 bp (β-actin). Lane M: DNA marker DL 2000, Lane 1: experimental group, Lane 2: vector control, Lane 3: mock control.

Figure 5 Weak expression of MMP-2 mRNA in experimental group but strong expression in vector andmock control shown by RT-PCR. The expected length of PCR products was 473 bp (MMP-2) and 205 bp (β-actin). Lane M: DNA marker DL 2000, Lane 1: experimental group, Lane 2: vector control, Lane 3: mock control.

Figure 6 Significant inhibition of VEGF mRNA and MMP-2 mRNAin experimental group comparedwith vector control and mock control shown by quantification analysis of PCR products of VEGF and MMP-2 mRNA on 1. 5% agarose gel containing ethidiumbromide (P < 0.05).Valueswere expressed asmean ± SD.

Figure 7 Location of immunohistochemical staining of expression of VEGF in cytoplasm and membrane. A: experimental group, B: vector control, C: mock control.

Figure 8 Down-regulation of VEGF protein expression by SSTR2. The expression of VEGF was significantly decreased in experimental group compared with vector control and mock control (P < 0.05). Values were expressed as mean ± SD.

Figure 9 Location of immunohistochemical staining of expression of MMP-2 in cytoplasm and membrane. A: experimental group, B: vector control, C: mock control (SABC × 400).

Figure 10 Down-regulation of MMP-2 protein expression by SSTR2. The expression of MMP-2 was significantly decreased in experimental group compared with vector control and mock control (P < 0.05). Values were expressed as mean ± SD.