Viral Hepatitis
Copyright ©The Author(s) 2004.
World J Gastroenterol. Oct 15, 2004; 10(20): 2967-2971
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.2967
Figure 1
Figure 1 Design of siRNAs specific for HBV genome. A: Down-ward arrows show the target sites within the RNA transcripts. The HBV ORFs are shown below aligned with HBV mRNA. B: Schematic presentation of U6 promoter constructs. The in-verted 19 nt sequences are separated by a 9 nt loop, transcrip-tion ends with 5Ts. The resulting shRNA (short hairpin RNA) are predicted to fold back to form a hairpin RNA.
Figure 2
Figure 2 Effect of RNAi on HBV protein expression in tran-siently transfected Huh-7 cells.
Figure 3
Figure 3 Effect of RNAi on HBV gene transcription detected by Northern blot. A: Northern blot experiment of HBV transcripts. The 28 s and 18s rRNAs were visualized under ultraviolet light for equal loading control. B: The hybridiza-tion signal was quantitatively evaluated by SmartviewTM im-age analysis software (Shanghai Fu-ri Inc).
Figure 4
Figure 4 Effect of RNAi on IFN-induced genes. Real-time PCR quantification of MxA and 2’-5’ OAS. The copy number of MxA and 2’-5’OAS were normalized with GAPDH. The rela-tive fold induction of each gene compared with mock trans-fection is shown.