Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoning

Figure 1 Identification of recombinant plasmids digested with restriction enzymes BamH I and Sal I. 1, 4, 5: pEGFP-HGF plas-mid digested with BamH I and Sal I; 2: pBS-7 plasmid digested with BamH I+Sal I; 3: λ DNA/HindIII marker.

Figure 2 Green fluorescence in QZG cells transfected with pEGFP-HGF fusion constructs, Inverted fluorescent light mi-croscope × 200.

Figure 3 Expression of HGF protein in QZG cells transfected with pEGFP-HGF. SABC × 400.

Figure 4 Relative values of ³H-TdR incorporation in QZG cells transferred with pEGFP-HGF (relative to QZG cells trans-ferred with pEGFP).

Figure 5 Expression of PCNA protein in QZG cells trans-ferred with pEGFP-HGF (A) and transferred with pEGFP (B). × 200.