Selection of a peptide mimicking neutralization epitope of hepatitis E virus with phage peptide display technology

Figure 1 Expression vector construction of pC149-mut-8C11A and pC149-mut-8H3A. A: Construction of pC149-mut-8C11A, B: Map of pC149-mut-8H3A, which was constructed as pC149-mut-8C11A except using the primer 8H3AFP instead of 8C11AFP.

Figure 2 Sequences of peptides selected by monoclonal antibodies. A: Selected by mAb 8C11, B: Selected by mAb 8H3.

Figure 3 Expression and Western blotting of pC149-mut-8C11A and pC149-mut-8H3A. A, C: SDS-PAGE, B, D: Western blot-ting of mAbs 8C11 and 8H3 respectively, A, B: pC149-mut-8C11A, C, D: pC149-mut-8H3A. 1, Protein molecular weight marker; 2, Supernatant of ultrasonic lysate; 3, Deposition of ultrasonic lysate; 4, C149-mut control; 5, Purified NE2 antigen

Figure 4 Virus like particles assemblied by recombinant protein C8C11A, C8H3A or C149 mut (Negative staining electron microscopy, × 100000).

Figure 5 Binding curve of chemo-synthesized seven peptide 8H3A against monoclonal antibody 8H3 in BIAcore biosensor.