Expression and altered subcellular localization of the cyclin-dependent kinase inhibitor p27Kip1 in hepatocellular carcinoma

Figure 1 Negative control of p27 (× 400).

Figure 2 Positive control of p27 (× 400).

Figure 3 Immunohistochemical staining of p27 in different liver tissues (Elivision), A: p27 expression is decreased in HCC × 400, B: Decreased p27 expression in HCC (right), the p27 expression is mostly located in the cytoplasm in nontumoral liver tissues (left) × 200, C: Some tumor cells show cytoplasmic staining of p27 × 400, D: p27 staining in normal controls is mostly located in nuclear × 400.

Figure 4 Expression of PCNA in different liver tissues (Elivision), A: Expression of PCNA in HCC × 400, B: Expression of PCNA in HCC (left) and in nontumoral liver tissues (right) × 200, C: Expression of PCNA in nontumoral liver tissues × 400, D: in normal controls × 400.

Figure 5 Kaplan-Meier curve for actuarial survival of patients in low p27 and high p27 groups.