Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 7, 2021; 27(29): 4784-4801
Published online Aug 7, 2021. doi: 10.3748/wjg.v27.i29.4784
Hepatitis B virus infection modeling using multi-cellular organoids derived from human induced pluripotent stem cells
Di Cao, Jian-Yun Ge, Yun Wang, Tatsuya Oda, Yun-Wen Zheng
Di Cao, Yun Wang, Yun-Wen Zheng, Institute of Regenerative Medicine and Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Jian-Yun Ge, Tatsuya Oda, Yun-Wen Zheng, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Ibaraki, Japan
Jian-Yun Ge, Yun-Wen Zheng, Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, and School of Biotechnology and Heath Sciences, Wuyi University, Jiangmen 529020, Guangdong Province, China
Yun-Wen Zheng, School of Medicine, Yokohama City University, Yokohama 234-0006, Kanagawa, Japan
Author contributions: Zheng YW conceptualized and designed this study, and conceived the writing; Cao D and Ge JY collected data and drafted the manuscript; Ge JY, Cao D, Wang Y and Zheng YW reviewed, discussed and revised the manuscript; all authors approved the final manuscript; Cao D and Ge JY contributed equally to this work; Zheng YW and Oda T are senior authors and co-correspondents of this work.
Supported by National Natural Science Foundation of China, No. 82070638 and No. 81770621; and JSPS KAKENHI, No. JP18H02866.
Conflict-of-interest statement: The authors declare no potential financial interests.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Yun-Wen Zheng, PhD, Associate Professor, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Faculty of Medicine, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8575, Ibaraki, Japan.
Received: March 9, 2021
Peer-review started: March 9, 2021
First decision: April 17, 2021
Revised: April 30, 2021
Accepted: July 15, 2021
Article in press: July 15, 2021
Published online: August 7, 2021
Core Tip

Core Tip: The development of an effective treatment for hepatitis B virus relies on reliable and reproducible in vitro modeling systems. Recently, three-dimensional multi-cellular organoid systems have attracted considerable attention owing to their superior susceptibility and capability to precisely recapitulate the virus life cycle. Recent advances in organoid-generating strategies, particularly those derived from human induced pluripotent stem cells, together with future improvements in genetic modification and scalability, will undoubtedly promote personalized disease modeling and drug development.