Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 7, 2021; 27(17): 1973-1992
Published online May 7, 2021. doi: 10.3748/wjg.v27.i17.1973
S100 calcium binding protein A6 and associated long noncoding ribonucleic acids as biomarkers in the diagnosis and staging of primary biliary cholangitis
Xi-Hua Dong, Di Dai, Zhi-Dong Yang, Xiao-Ou Yu, Hua Li, Hui Kang
Xi-Hua Dong, Di Dai, Zhi-Dong Yang, Xiao-Ou Yu, Hua Li, Hui Kang, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Author contributions: All authors participated in the design, interpretation of the studies, analysis of the data and review of the manuscript; Dong XH wrote the manuscript; Dong XH, Dai D and Yang ZD conducted the experiment; Yu XO and Li H collected data; Dong XH, Yu XO and Li H analyzed the data; the article was approved by Kang H; The final version of the article was approved by all authors.
Supported by National Natural Science Foundation of China, No. 81871723.
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of The First Affiliated Hospital of China Medical University (Approval No. 2018-89-2).
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals [China Medical University Application for Laboratory Animal Welfare and Ethical review (201702 Edition)].
Conflict-of-interest statement: The authors declare that there are no conflicts of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Hui Kang, PhD, Professor, Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, No. 155 Nanjing North Street, Shenyang 110001, Liaoning Province, China.
Received: December 7, 2020
Peer-review started: December 7, 2020
First decision: January 10, 2021
Revised: January 23, 2021
Accepted: March 10, 2021
Article in press: March 10, 2021
Published online: May 7, 2021
Core Tip

Core Tip: Primary biliary cholangitis (PBC) is an autoimmune liver disease which is characterized by intrahepatic cholestasis. The expression of S100 calcium binding protein A6 (S100A6) was up-regulated in a bile duct ligation mouse model compared with sham mice. The relative expression levels of plasma S100A6 messenger ribonucleic acid, LINC00472 and LINC01257 were up-regulated and the relative expression of LINC00312 was down-regulated in PBC patients. S100A6 and the three long noncoding ribonucleic acids can be used as biomarkers for PBC diagnosis and staging using receiver operating characteristic curve analysis. The results were further verified in vitro using intrahepatic biliary epithelial cells.