Metabolite profile comparisons between ascending and descending colon tissue in healthy adults
Bridget A Baxter, Kristopher D Parker, Michael J Nosler, Sangeeta Rao, Rebecca Craig, Catherine Seiler, Elizabeth P Ryan
Bridget A Baxter, Kristopher D Parker, Elizabeth P Ryan, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Fort Collins, CO 80523, United States
Michael J Nosler, University of Colorado Health Gastroenterology Clinic, Fort Collins, CO 80524, United States
Sangeeta Rao, Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, United States
Rebecca Craig, Harmony Surgery Center, Fort Collins, CO 80528, United States
Catherine Seiler, Director of Clinical Operations, Harmony Surgery Center, Fort Collins, CO 80523, United States
Author contributions: Ryan EP and Nosler MJ designed research and maintained study oversight; Nosler MJ, Craig R and Seiler C conducted human tissue sample collections; Baxter BA and Parker KD analyzed the metabolite profile and microbiome data collected from stool and colon; Parker KD and Rao S performed statistical analysis; Baxter BA, Parker KD, and Ryan EP wrote the manuscript; Ryan EP had primary responsibility and all authors approved the final product.
Supported by the CancerCure Foundation and the University of Colorado Cancer Center Support Grant, Division of Cancer Control and Prevention (Aurora, CO), No. P30CA046934.
Institutional review board statement: Colorado State University Institutional Review Board No. 15-6051, and University Colorado Health-North Institutional Review Board No. 0010144.
Informed consent statement: Participants provided written informed consent.
Conflict-of-interest statement: Authors have nothing to disclose.
Data sharing statement: Metataxonomics sequence data are available via NCBI SRA BioProject Accession PRJNA594611 and on this project’s GitHub repository github.com/kdprkr/ConjurersBrew.
STROBE statement: The authors have read the STROBE guidelines, and the manuscript was prepared and revised according to the STROBE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Elizabeth P Ryan, PhD, Associate Professor, Department of Environmental and Radiological Health Sciences, Colorado State University, 1617 Campus Delivery, Fort Collins, CO 80523, United States. e.p.ryan@colostate.edu
Received: October 22, 2019
Peer-review started: October 15, 2019
First decision: November 13, 2019
Revised: December 11, 2019
Accepted: December 21, 2019
Article in press: December 21, 2019
Published online: January 21, 2020
