Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor
Sung-Hoon Han, Sehwan Shim, Min-Jung Kim, Hye-Yun Shin, Won-Suk Jang, Sun-Joo Lee, Young-Woo Jin, Seung-Sook Lee, Seung Bum Lee, Sunhoo Park, Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, Seoul 01812, South Korea
Author contributions: Lee SB and Park S contributed equally to this work; Han SH performed the majority of experiments and analyzed the data; Shim S, Kim MJ, Shin HY and Jang WS participated in care/treatment of animals and performed the molecular biochemical investigations; Lee SJ, Jin YW and Lee SS designed and coordinated the research; Lee SB and Park S analyzed the data and wrote paper; all the authors contributed to this manuscript.
Supported by a grant of the Korea Institute of Radiological and Medical Sciences, funded by Ministry of Science, ICT and Future Planning, South Korea, No. 1711031810/50586-2016 and No. 1711031808/50581-2016.
Institutional review board statement: The study was revised and approved by the Korea Institute of Radiological and Medical Sciences Institutional Review Board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Korea Institute of Radiological and Medical Sciences in Korea (IACUC protocol number: kirams2016-0043).
Conflict-of-interest statement: The authors declare that they have no potential conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Sunhoo Park, MD, PhD, Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul 01812, South Korea. email@example.com
Telephone: +82-2-9701274 Fax: +82-2-9702430
Received: September 5, 2016
Peer-review started: September 6, 2016
First decision: October 20, 2016
Revised: November 3, 2016
Accepted: November 23, 2016
Article in press: November 23, 2016
Published online: February 14, 2017