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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 28, 2017; 23(44): 7830-7839
Published online Nov 28, 2017. doi: 10.3748/wjg.v23.i44.7830
Published online Nov 28, 2017. doi: 10.3748/wjg.v23.i44.7830
Composition and immuno-stimulatory properties of extracellular DNA from mouse gut flora
Ce Qi, Jin Sun, The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province, China
Ce Qi, Ya Li, Xing-Guo Wang, Guo-wei Le, Jin Sun, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China
Ren-Qiang Yu, Wuxi Maternal and Child Health Hospital, Wuxi 212422, Jiangsu Province, China
Sheng-Li Zhou, Quality of Research and Development Department, COFCO Fortune Food Sales & Distribution Co., Ltd. Tianjin 300452, China
Hang Xiao, Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States
Author contributions: Sun J and Li Y designed the research; Qi C, Li Y, Wang XG, Le GW, and Zhou SL performed the research; Sun J, Li Y, Yu RQ, and Xiao H analyzed the data and wrote the article; Xiao H and Yu RQ revised the paper; all authors have read and approved the final version to be published.
Supported by China Postdoctoral Science Foundation , No. 172774 ; Fund of Key Laboratory of Carbohydrate Chemistry and Biotechnology , Ministry of Education, Jiangnan University, No. KLCCB-KF201603 ; and National Natural Science Foundation of China , No. 31201805 .
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Jiangnan University (IACUC protocol number: No. 8/2014/JU).
Conflict-of-interest statement: No potential conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Jin Sun, PhD, Associate Professor, School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China. sunj@jiangnan.edu.cn
Telephone: +86-510-85917780
Received: September 22, 2017
Peer-review started: September 23, 2017
First decision: October 11, 2017
Revised: October 14, 2017
Accepted: October 27, 2017
Article in press: October 27, 2017
Published online: November 28, 2017
Peer-review started: September 23, 2017
First decision: October 11, 2017
Revised: October 14, 2017
Accepted: October 27, 2017
Article in press: October 27, 2017
Published online: November 28, 2017
Core Tip
Core tip: Our results revealed that degraded bacterial genomic DNA was mainly released by Gram-negative bacteria, especially Bacteroidales-S24-7 and Stenotrophomonas genus in gut mucus of mice. They decreased pro-inflammatory activity compared to genomic DNA of total gut flora. Our study highlights that bacteria derived DNA plays an important role in modulating local immune response in mouse gut.