Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 21, 2017; 23(43): 7678-7692
Published online Nov 21, 2017. doi: 10.3748/wjg.v23.i43.7678
Resveratrol modifies biliary secretion of cholephilic compounds in sham-operated and cholestatic rats
Eva Dolezelova, Alena Prasnicka, Jolana Cermanova, Alejandro Carazo, Lucie Hyrsova, Milos Hroch, Jaroslav Mokry, Michaela Adamcova, Alena Mrkvicova, Petr Pavek, Stanislav Micuda
Eva Dolezelova, Department of Biological and Medical Sciences, Charles University, Faculty of Pharmacy in Hradec Kralove, 50003 Hradec Kralove, Czech Republic
Alena Prasnicka, Jolana Cermanova, Stanislav Micuda, Department of Pharmacology, Charles University, Faculty of Medicine in Hradec Kralove, 50003 Hradec Kralove, Czech Republic
Alejandro Carazo, Lucie Hyrsova, Petr Pavek, Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, 50003 Hradec Kralove, Czech Republic
Milos Hroch, Alena Mrkvicova, Department of Medical Biochemistry, Charles University, Faculty of Medicine in Hradec Kralove, 50003 Hradec Kralove, Czech Republic
Jaroslav Mokry, Department of Histology and Embryology, Charles University, Faculty of Medicine in Hradec Kralove, 50003 Hradec Kralove, Czech Republic
Michaela Adamcova, Department of Physiology, Charles University, Faculty of Medicine in Hradec Kralove, 50003 Hradec Kralove, Czech Republic
Author contributions: Dolezelova E and Micuda S analyzed the data; Dolezelova E and Micuda S wrote the manuscript; Dolezelova E, Prasnicka A, Cermanova J, Hroch M, Mokry J, Adamcova M, Mrkvicova A and Micuda S performed the in vivo experiments and all molecular analyses; Carazo A, Hyrsova L and Pavek P contributed to the in vitro experiments; All authors participated in the revision and approved the final manuscript.
Supported by Grant Agency of Charles University, No. Progres Q40/05; Specific University Research, No. SVV-2016-260287; and Czech Science Foundation (GA CR), No. 303/12/G163.
Institutional review board statement: The study was reviewed and approved by the Institutional Animal Care and Use Committee of Charles University, Faculty of Medicine in Hradec Kralove.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Charles University in Prague, Faculty of Medicine in Hradec Kralove (3163/2008-30).
Conflict-of-interest statement: The authors declare that they have no conflict of interest related to this study.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Stanislav Micuda, MD, PhD, Professor, Department of Pharmacology, Charles University, Faculty of Medicine in Hradec Kralove, Simkova 870, 50003 Hradec Kralove, Czech Republic. micuda@lfhk.cuni.cz
Telephone: +420-495-816233 Fax: +420-495-513022
Received: June 30, 2017
Peer-review started: June 30, 2017
First decision: July 25, 2017
Revised: August 15, 2017
Accepted: September 5, 2017
Article in press: September 5, 2017
Published online: November 21, 2017
Core Tip

Core tip: For the first time, our results provide information about the ability of resveratrol to increase bile flow in healthy rats by increasing the biliary excretion of bile acids and glutathione via posttranscriptional induction of their rate-limiting transporters, Bsep and Mrp2, respectively, and via the up-regulation of Cyp7a1, an enzyme that is crucial for bile acid synthesis. Resveratrol simultaneously induced hepatic expression of Mdr1, which was verified by increased biliary excretion of its substrate, azithromycin. Our findings were consistent with an agonistic effect of resveratrol on PXR. Our data therefore imply that oral administration of resveratrol may modify the kinetics of endo- and xenobiotics.