Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 14, 2017; 23(42): 7584-7593
Published online Nov 14, 2017. doi: 10.3748/wjg.v23.i42.7584
Diversity of bacterial lactase genes in intestinal contents of mice with antibiotics-induced diarrhea
Cheng-Xing Long, Lu He, Yan-Fang Guo, Ya-Wei Liu, Nen-Qun Xiao, Zhou-Jin Tan
Cheng-Xing Long, Lu-He, Yan-Fang Guo, Ya-Wei Liu, Nen-Qun Xiao, Zhou-Jin Tan, Department of Microbiology, Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China
Cheng-Xing Long, College of Mathematics and Finance, Hunan University of Humanities, Science and Technology, Loudi 417000, Hunan Province, China
Author contributions: Tan ZJ designed the study; He L and Guo YF performed the experiments; Liu YW and Long CX analyzed the data; Long CX and Xiao NQ wrote the paper.
Supported by the National Natural Science Foundation of China, No. 81573951.
Institutional review board statement: The study was approved by the Institutional Review Board of Hunan University of Chinese Medicine, Changsha, China.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Animal Ethics and Welfare Committee of Hunan University of Chinese Medicine, Animal License SCXK (Xiang) 2013-0004.
Conflict-of-interest statement: The authors declare no conflict of interest related to this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Zhou-Jin Tan, PhD, Professor, Department of Microbiology, Hunan University of Chinese Medicine, 300 Xueshi Road, Changsha 410208, Hunan Province, China.
Telephone: +86-13974954942
Received: July 16, 2017
Peer-review started: July 18, 2017
First decision: August 30, 2017
Revised: September 14, 2017
Accepted: September 26, 2017
Article in press: September 26, 2017
Published online: November 14, 2017
Core Tip

Core tip: The mechanism of antibiotics-induced diarrhea has been studied in a wide range of diverse microbes, but less on functional enzymes. The current study aimed to determine the mechanism of lactase activity from genetic diversity and provide a basis for antibiotics-induced diarrhea. Alpha/Beta diversity analysis showed that there were significant differences between the control mice and model mice in types of lactase genes expressed and their activities. Following the antibiotics-induced diarrhea symptoms, the intestinal lactase genes changed, the number of strains was reduced and the abundance decreased, indicating changes in community structure and decreased diversity of lactase genes.