Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR

doi:10.3748/wjg.v23.i39.7087

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2017; 23(39): 7087-7097
Published online Oct 21, 2017. doi: 10.3748/wjg.v23.i39.7087

Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR

Susana Olmedillas-López, Dennis César Lévano-Linares, Carmen Laura Aúz Alexandre, Luz Vega-Clemente, Edurne León Sánchez, Alejandro Villagrasa, Jaime Ruíz-Tovar, Mariano García-Arranz, Damián García-Olmo

Susana Olmedillas-López, Luz Vega-Clemente, Alejandro Villagrasa, Mariano García-Arranz, Damián García-Olmo, Foundation Health Research Institute-Fundación Jiménez Díaz University Hospital, Madrid 28040, Spain

Dennis César Lévano-Linares, Mariano García-Arranz, Damián García-Olmo, Department of Surgery, School of Medicine, Universidad Autónoma de Madrid, Madrid 28029, Spain

Dennis César Lévano-Linares, Jaime Ruíz-Tovar, Department of Surgery, Rey Juan Carlos University Hospital, Madrid 28933, Spain

Carmen Laura Aúz Alexandre, Department of Pathology, Fundación Jiménez Díaz University Hospital, Madrid 28040, Spain

Edurne León Sánchez, Department of Biomedicine and Biotechnology, Universidad de Alcalá, Madrid 28805, Spain

Damián García-Olmo, Department of Surgery, Fundación Jiménez Díaz University Hospital, Madrid 28040, Spain

Author contributions: Olmedillas-López S and Lévano-Linares DC contributed equally to this work; Olmedillas-López S, Lévano-Linares DC, García-Arranz M and García-Olmo D conceived and designed the experiments; Olmedillas-López S, Aúz Alexandre CL, Vega-Clemente L, León Sánchez E and Villagrasa A performed the experiments; Olmedillas-López S, Lévano-Linares DC, Ruíz-Tovar J, García-Arranz M and García-Olmo D analyzed the data; Olmedillas-López S and Lévano-Linares DC wrote the paper.

Supported by “Fondo de Investigaciones Sanitarias (FIS)-FEDER”, Ministry of Health, Spain, No. PI13/01924 to García-Olmo D; and RETIC Program of ISCIII-FEDER, No. RD12/0019/0035 to Olmedillas-López S.

Institutional review board statement: This study was reviewed and approved by the Institutional Ethics Committee for Clinical Research of the Fundación Jiménez Díaz University Hospital (FJD) (PIC 63/2016_FJD).

Conflict-of-interest statement: The authors have no conflict of interest to declare.

Data sharing statement: Individual participant consent was not obtained for data sharing but the presented data are anonymized and there is no possibility of identification. No additional data are available.

Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

Correspondence to: Susana Olmedillas-López, PhD, Postdoc Researcher, Foundation Health Research Institute-Fundación Jiménez Díaz University Hospital, Avda. Reyes Católicos 2, Madrid 28040, Spain. susana.olmedillas@fjd.es

Telephone: +34-91-5504800-2781 Fax: +34-91-5505353

Received: June 28, 2017
Peer-review started: June 28, 2017
First decision: August 15, 2017
Revised: September 15, 2017
Accepted: September 26, 2017
Article in press: September 26, 2017
Published online: October 21, 2017

Core Tip

Core tip: The potential of droplet digital PCR (ddPCR) to detect KRAS G12D mutation in stool DNA from colorectal cancer (CRC) patients was examined as a proof-of-concept for the applicability of this technology to study DNA biomarkers in stool-derived DNA. It was shown that KRAS G12D detection in stool-derived DNA from CRC patients by ddPCR is feasible and provides comparable results to the analysis of formalin-fixed paraffin-embedded tissue by pyrosequencing. These results suggest that analysis of KRAS mutations and other molecular biomarkers in stool by ddPCR could represent a complementary non-invasive approach to standard screening tests for CRC.