Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 7, 2017; 23(29): 5324-5332
Published online Aug 7, 2017. doi: 10.3748/wjg.v23.i29.5324
MicroRNA exhibit altered expression in the inflamed colonic mucosa of ulcerative colitis patients
Swati Valmiki, Vineet Ahuja, Jaishree Paul
Swati Valmiki, Jaishree Paul, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
Vineet Ahuja, Department of Gastroenterology, All India Institute of Medical Sciences, New Delhi 110029, India
Author contributions: Valmiki S and Paul J designed the study, analyzed the data and wrote the manuscript; Valmiki S performed the experiments; Ahuja V provided the samples with clinical history.
Supported by Department of Biotechnology, Ministry of Science and Technology, New Delhi, Government of India vide BT/PR8348/MED/30/1023/2013 to Paul J; PURSE grant from the Department of Science and Technology, New Delhi India vide 6(54) SLS/JP/DST PURSE/2015-2016.
Institutional review board statement: The study has been ethically approved from the Institute Ethics Committee, All India Institute of Medical Sciences (Ref. No. T-290/23.06.2015, RT-7/27.01.2016) and Institutional Ethics Review Board, JNU (IERB Ref. No.2016/Student/93).
Conflict-of-interest statement: The authors declare no conflict of interest in the present study.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Correspondence to: Dr. Jaishree Paul, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Telephone: +91-11-26704156 Fax: +91-11-26742558
Received: February 7, 2017
Peer-review started: February 8, 2017
First decision: March 30, 2017
Revised: May 22, 2017
Accepted: June 1, 2017
Article in press: June 1, 2017
Published online: August 7, 2017
Core Tip

Core tip: In order to get an insight into the pathogenesis of ulcerative colitis (UC), we explored spatial expression of microRNA in the inflamed and non-inflamed region of mucosal tissue of patients. Profiling of differentially expressed miRNA was generated by microarray from three paired samples. Few significantly dysregulated microRNA were validated using qRT-PCR. The present study reports disease specific alteration in the expression of miR-125b, miR-155, miR-223 and miR-138 in UC patients and also analyzed their biological significance using in silico tools. MiR-223 exhibited elevated expression independent of disease activity therefore, could be a potential candidate as biomarker for UC.