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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 21, 2016; 22(19): 4716-4731
Published online May 21, 2016. doi: 10.3748/wjg.v22.i19.4716
Published online May 21, 2016. doi: 10.3748/wjg.v22.i19.4716
Plasma long noncoding RNA expression profile identified by microarray in patients with Crohn’s disease
Dong Chen, Hui-Ying Zhao, Department of Colorectal Surgery, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Jiang Liu, Department of Gastroenterology, Huzhou Central Hospital, Huzhou 310003, Zhejiang Province, China
Yi-Peng Chen, Zun Xiang, Xi Jin, Department of Gastroenterology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Author contributions: Chen D and Liu J performed the majority of experiments; Zhao HY, Chen YP and Xiang Z participated in clinical sample collection, microarray, qRT-PCR and data analysis; Jin X designed the study and wrote the manuscript; Liu J and Jin X contribute equally as the first author.
Supported by National Natural Science Foundation of China, No. 81370008 and No. 81000169; Natural Science Foundation of Zhejiang Province, No. R2110159, No. LY15H030006 and No. LY16H030003; and Science Technology Project of Zhejiang Province, No. 2014C33205.
Institutional review board statement: This study was approved by the institution review board of the First Affiliated Hospital of Zhejiang University and conducted in accordance with the Declaration of Helsinki.
Conflict-of-interest statement: There are no conflicts of interest or commercial financial support for this manuscript.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Xi Jin, PhD, Department of Gastroenterology, the First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China. jxfl007@zju.edu.cn
Telephone: +86-571-87236611 Fax: +86-571-87236611
Received: February 11, 2016
Peer-review started: February 13, 2016
First decision: March 7, 2016
Revised: March 10, 2016
Accepted: March 30, 2016
Article in press: March 30, 2016
Published online: May 21, 2016
Peer-review started: February 13, 2016
First decision: March 7, 2016
Revised: March 10, 2016
Accepted: March 30, 2016
Article in press: March 30, 2016
Published online: May 21, 2016
Core Tip
Core tip: The pathogenesis of Chrohn’s disease (CD) is unclear while increasing evidence supports the involvement of epigenomic regulation. In this study, we jointly used microarray screening and qRT-PCR verification to achieve the plasma specific long noncoding RNAs expression profile of patients with CD and their potential regulation of downstream mRNAs. Our results would provide preliminary data for non-invasive diagnosis of CD and a reservoir for specific lncRNA-mRNA pathway exploration in the future.