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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 21, 2016; 22(15): 3978-3991
Published online Apr 21, 2016. doi: 10.3748/wjg.v22.i15.3978
Published online Apr 21, 2016. doi: 10.3748/wjg.v22.i15.3978
Mir-30d increases intracellular survival of Helicobacter pylori through inhibition of autophagy pathway
Xiao-Jun Yang, Ruo-Huang Si, Bing-Qiang Ma, Ze-Bin Jiang, Bin Wang, Peng Gao, Department of General Surgery, Gansu Provincial Hospital, Lanzhou 730000, Gansu Province, China
Yu-He Liang, Department of General Surgery, The People’s Hospital of Baoji City, Baoji 721000, Shaanxi Province, China
Author contributions: Yang XJ, Si RH and Liang YH contributed equally to this work; Yang XJ and Liang YH carried out cell culture, transfection and the molecular genetic studies, drafted the manuscript; Si RH carried out the RT-PCR; Ma BQ and Jiang ZB participated in the GFP-LC3 Plasmid Transfection and Flow cytometry; Wang B performed the statistical analysis; and Gao P conceived of the study, and participated in its design and coordination and helped to draft the manuscript.
Supported by the National Natural Science Fund from China, No. 81260326.
Institutional review board statement: This study was reviewed and approved by Gansu Provincial Hospital Institutional review board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional animal care and use committee of Gansu Provincial Hospital.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Peng Gao, MD, Professor, Chief, Department of General Surgery, Gansu Provincial Hospital, No. 204 Donggang West Road, Lanzhou 730000, Gansu Province, China. gaopenglz@aliyun.com
Telephone: +86-931-8281015 Fax: +86-931-8266957
Received: December 7, 2015
Peer-review started: December 10, 2015
First decision: December 30, 2015
Revised: January 14, 2016
Accepted: February 20, 2016
Article in press: February 22, 2016
Published online: April 21, 2016
Peer-review started: December 10, 2015
First decision: December 30, 2015
Revised: January 14, 2016
Accepted: February 20, 2016
Article in press: February 22, 2016
Published online: April 21, 2016
Core Tip
Core tip: In this study, we tested a hypothesis that mir-30d could repress autophagy in response to Helicobacter pylori (H. pylori) invasion by directly targeting multiple core genes of the autophagy pathway, including ATG2B, ATG5, ATG12, BECN1 and BNIP3L in gastric epithelial cells. Inhibition of autophagy increased the intracellular survival of H. pylori in AGS cells, and the repression of autophagy by mir-30d may help the intracellular H. pylori to evade autophagic clearance. These findings provide a novel mechanism for elucidating persistent H. pylori infection and provide a promising target for gastric cancer prevention.