Original Article
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World J Gastroenterol. Aug 14, 2014; 20(30): 10440-10448
Published online Aug 14, 2014. doi: 10.3748/wjg.v20.i30.10440
Osteopontin knockdown suppresses the growth and angiogenesis of colon cancer cells
Xin-Lin Wu, Kai-Jin Lin, Ai-Ping Bai, Wan-Xiang Wang, Xing-Kai Meng, Xiu-Lan Su, Ming-Xing Hou, Pei-De Dong, Jun-Jing Zhang, Zhao-Yang Wang, Lin Shi
Xin-Lin Wu, Wan-Xiang Wang, Xing-Kai Meng, Ming-Xing Hou, Pei-De Dong, Jun-Jing Zhang, Zhao-Yang Wang, Department of General Surgery, the Affiliated Hospital of Inner Mongolia Medical University, Huhhot 010059, Inner Mongolian Autonomous Region, China
Kai-Jin Lin, Department of Internal Medicine, Fuqing City Hospital, Fuqing 350300, Fujian Province, China
Ai-Ping Bai, Department of Gastroenterology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
Xiu-Lan Su, Clinical Medicine Research Center, the Affiliated Hospital of Inner Mongolia Medical University, Huhhot 010059, Inner Mongolian Autonomous Region, China
Lin Shi, Department of Pathology, the Affiliated Hospital of Inner Mongolia Medical University, Huhhot 010059, Inner Mongolian Autonomous Region, China
Author contributions: Wu XL and Lin KJ contributed equally to this work; Wu XL, Bai AP, Meng XK and Shi L designed the research; Lin KJ, Wang WX, Meng XK, Su XL, Hou MX, Dong PD, Zhang JJ, Wang ZY and Shi L performed the research and analyzed the data; Wu XL, Bai AP and Shi L wrote the paper.
Supported by Grants from the National Natural Science Foundation of China, No. 81260364, No. 81270472 and No. 81070310; the “Chunhui” Program of Ministry of Education in China, No. Z2012007; and the Natural Science Foundation of Inner Mongolian Autonomous Region, No. 2012MS1123 and No. 2013MS1132
Correspondence to: Lin Shi, MD, Department of Pathology, the Affiliated Hospital of Inner Mongolia Medical University, 1 Tongdao North Street, Inner Mongolian Autonomous Region, Huhhot 010059, Inner Mongolian Autonomous Region, China. shilinsd2003@aliyun.com
Telephone: +86-471-6637678 Fax: +86-471-6637678
Received: January 5, 2014
Revised: March 28, 2014
Accepted: April 28, 2014
Published online: August 14, 2014
Core Tip

Core tip: This study investigated the anti-tumor effects of osteopontin (OPN) knockdown in human colon cancer (Lovo) cells. Despite recent evidence that shows OPN expression is closely related to the development of colorectal cancer, the mechanism remains elusive. The results of the present study showed that siRNA-mediated downregulation of OPN inhibited the expression of vascular endothelial growth factor, matrix metalloproteinase (MMP)-2, MMP-9 and urokinase plasminogen activator in Lovo cells, which may lead to decreased invasion and angiogenesis of colon cancer.