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World J Gastroenterol. May 14, 2014; 20(18): 5474-5482
Published online May 14, 2014. doi: 10.3748/wjg.v20.i18.5474
Published online May 14, 2014. doi: 10.3748/wjg.v20.i18.5474
Use of lectin microarray to differentiate gastric cancer from gastric ulcer
Wei-Li Huang, Hui-Fan Ji, Bao-Rong Chi, Department of Hepatology, the First Clinic Hospital of Jilin University, Changchun 130021, Jilin Province, China
Wei-Li Huang, Xiao-Hui Guan, Department of Gastroenterology, Affiliated Hospital of Beihua University, Jilin 132001, Jilin Province, China
Yang-Guang Li, Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China
Yong-Chen Lv, Department of General Surgery, the Central Hospital of Jilin City, Jilin 132001, Jilin Province, China
Author contributions: Huang WL performed all the experiments; Li YG did all the statistical analysis; Lv YC and Guan XH contributed on the animal preparation; Ji HF contributed on some of the histological experiments; Chi BR designed the experiments and analyzed the data.
Correspondence to: Bao-Rong Chi, MD, PhD, Professor, Department of Hepatology, the First Clinic Hospital of Jilin University, No. 71 Xinmin Dajie, Changchun 130021, Jilin Province, China. chibr@jlu.edu.cn
Telephone: +86-431-88782528 Fax: +86-431-85612468
Received: October 13, 2013
Revised: January 26, 2014
Accepted: March 6, 2014
Published online: May 14, 2014
Revised: January 26, 2014
Accepted: March 6, 2014
Published online: May 14, 2014
Core Tip
Core tip: To assess the different glycopatterns in gastric ulcer and cancer by lectin microarray, which was then validated by lectin histochemistry. The results showed that the glycosylation level of gastric cancer was significantly higher than that in ulcer; the subsequent validation with two lectins (MPL and VVA) using lectin histochemistry showed higher positive rates and signal intensity in all types of gastric cancer detected, when compared with ulcer, which was consistent with the results of the lectin microarray, suggesting that GalNAc bound by the lectins MPL and VVA could be used to distinguish gastric cancer from ulcer.