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World J Gastroenterol. Sep 28, 2013; 19(36): 6044-6054
Published online Sep 28, 2013. doi: 10.3748/wjg.v19.i36.6044
Published online Sep 28, 2013. doi: 10.3748/wjg.v19.i36.6044
In vitro effect of amoxicillin and clarithromycin on the 3’ region of cagA gene in Helicobacter pylori isolates
Javier Andrés Bustamante-Rengifo, Andrés Januer Matta, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, 760043 Cali, Colombia
Alvaro Pazos, Department of Biology, Universidad de Nariño, 520002 San Juan de Pasto, Colombia
Author contributions: All the authors were involved in the acquisition and interpretation of the results, read and approved the final manuscript; Bustamante-Rengifo JA, Matta AJ and Pazos A conducted the microbiological and molecular tests; Bustamante-Rengifo JA analyzed the data; Bustamante-Rengifo JA and Bravo LE wrote, edited, and revised the manuscript.
Supported by The Administrative Department on Science and Innovation of the Republic of Colombia-COLCIENCIAS, No. RC-1106-408-20549, RC-1106-493-26237; and its program: Jóvenes Investigadores e Innovadores “Virginia Gutiérrez de Pineda”, and by the Universidad del Valle, Cali, Colombia
Correspondence to: Javier Andrés Bustamante-Rengifo, MSc, Associate Researcher, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Street 4 B N0. 36-00, Building 116, Floor 4°, 760043 Cali, Colombia. javierandres.bustamante@gmail.com
Telephone: +57-2-3212100-4101 Fax: +57-2-6670329
Received: January 25, 2013
Revised: April 4, 2013
Accepted: May 7, 2013
Published online: September 28, 2013
Revised: April 4, 2013
Accepted: May 7, 2013
Published online: September 28, 2013
Core Tip
Core tip: This study evaluated the in vitro effect of amoxicillin and clarithromycin on the cag pathogenicity island (cag PAI). It was found that the effect of antibiotic pressure does not induce loss of cag PAI, but it can lead - in most cases - to genetic rearrangements (loss or gain of Glu-Pro-Ile-Tyr-Ala motif) within the 3’ region of cagA gene of the founding bacteria that can affect the level of tyrosine phosphorylation impacting on its cellular effects and lead to divergence of cagA-positive subclones, which as a set could alter the pathogenic process of Helicobacter pylori in cases with treatment failure.