Published online Feb 14, 2020. doi: 10.3748/wjg.v26.i6.670
Peer-review started: November 8, 2019
First decision: December 23, 2019
Revised: January 6, 2020
Accepted: January 11, 2020
Article in press: January 11, 2020
Published online: February 14, 2020
Esophageal carcinoma is a common digestive tract cancer, which frequently recurs after treatment. MiR-1304 is a newly discovered non-coding RNA, which shows differential expression in other cancers, but its clinical value in esophageal carcinoma remains unclear.
To identify potential diagnostic and prognostic indicators of esophageal cancer recurrence.
To determine the diagnostic and prognostic value of miR-1304 in esophageal carcinoma recurrence.
Data on miRs with potential differences in esophageal carcinoma were screened from the Cancer Genome Atlas. A quantitative real-time polymerase chain reaction was employed to determine the expression of miR-1304 in esophageal carcinoma patients, and the clinicopathological features of these patients were analyzed. Based on the analysis of screened data and the expression of miR-1304 in esophageal carcinoma patients, the function of miR-1304 was evaluated. Moreover, the patients were followed-up to analyze prognosis. Target genes of miR-1304 were predicted, and the functions of these genes were analyzed.
The expression of miR-1304 in the tissues and serum of patients increased, similar to that seen in the database. Patients with high expression of miR-1304 had increased rates of tumor ≥ 3 cm, low differentiation and stage II + III disease. MiR-1304 had diagnostic value in identifying esophageal carcinoma, tumor size, differentiation and TNM staging. Tumor size, differentiation, TNM staging, and miR-1304 were independent risk factors for recurrence of esophageal carcinoma, and had certain predictive and diagnostic value for recurrence of this disease. Patients with high expression of miR-1304-3p had a lower survival rate. Multivariate analysis revealed that tumor size, differentiation, recurrence and miR-1304 were independent factors for prognosis. Furthermore, the target genes had 18 functions with aP < 0.05 according to gene ontology enrichment analysis and 11 signal pathways with aP < 0.05 according to the Kyoto Encyclopedia of Genes and Genomes. In addition, there were 269 relationship pairs according to String analysis of protein co-expression, of which the co-expression pairs with epidermal growth factor were the most common.
MiR-1304 can be used as a potential indicator for the diagnosis and recurrence of esophageal carcinoma, and survival of patients.
In future research, the molecular mechanism of miR-1304 in esophageal carcinoma will be studied.