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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 21, 2020; 26(35): 5314-5327
Published online Sep 21, 2020. doi: 10.3748/wjg.v26.i35.5314
Published online Sep 21, 2020. doi: 10.3748/wjg.v26.i35.5314
Investigation of immune escape-associated mutations of hepatitis B virus in patients harboring hepatitis B virus drug-resistance mutations
Bi-Xia Huang, Xin-Guang Liu, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics/Institute of Biochemistry & Molecular Biology, Guangdong Medical University, Dongguan 523808, Guangdong Province, China
Yan Liu, Zhen-Ping Fan, Lan-Lan Si, Rong-Juan Chen, Jun Wang, Fu-Sheng Wang, Dong-Ping Xu, Institute of Infectious Diseases, the Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China
Dan Luo, Department of Infectious Diseases, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: Huang BX was involved in the experiments, procedures, statistical analysis, writing of the original draft, and critical revision of manuscript; Liu Y performed the experiments and procedures; Fan ZP took part in the statistical analysis; Si LL was involved in the experiments and procedures; Chen RJ performed the experiments and procedures; Wang J took part in the statistical analysis; Luo D was involved in the study statistical analysis; Wang FS performed the study statistical analysis; Xu DP took part in the study conception, design, writing of the original draft, and critical revision of manuscript; Liu XG was involved in the study conception, design, writing of the original draft, and critical revision of manuscript; all authors have read and approved the final manuscript.
Supported by the National Natural Science Foundation of China , No. 81572010, No. 81671399, No. 81721002 and No. 81971329 ; the Capital Health Research and Development of Special Fund Program , No. 2016-2-5032 ; and the Beijing Natural Science Foundation No. 7172206 .
Institutional review board statement: The study was reviewed and approved by the Ethics Committee Approval Document of 302 Hospital, Institutional Review Board Approval No. 2012020D; The study was reviewed and approved by the Ethics Committee Approval Document of 302 Hospital, Institutional Review Board Approval No. 2013052D.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: We do not have any patents, whether planned, pending or issued, broadly relevant to the work.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Dong-Ping Xu, MD, PhD, Professor, Institute of Infectious Diseases, the Fifth Medical Center of Chinese PLA General Hospital, No. 100 Xisihuan Middle Road, Beijing 100039, China. xudongping302@sina.com
Received: April 23, 2020
Peer-review started: April 23, 2020
First decision: May 1, 2020
Revised: July 27, 2020
Accepted: August 12, 2020
Article in press: August 12, 2020
Published online: September 21, 2020
Peer-review started: April 23, 2020
First decision: May 1, 2020
Revised: July 27, 2020
Accepted: August 12, 2020
Article in press: August 12, 2020
Published online: September 21, 2020
ARTICLE HIGHLIGHTS
Research background
A large number of patients were surveyed for both immune escape-associated and drug-resistance mutations.
Research motivation
A link between immune escape-associated and resistance mutations was identified.
Research objectives
The association between immune escape-associated mutations and nucleotide analog resistance mutations was evaluated.
Research methods
Upon follow-up, hepatitis B virus (HBV) sA159V was found to have contributed to resistance in several patients.
Research results
HBV sA159V reduced the hepatitis B surface antigen production but increased the replication capacity of lamivudine (LAM)/entecavir (ETV)-resistant mutants.
Research conclusions
sA159V might increase the fitness of LAM/ETV-resistant mutants under environmental pressure in some cases.
Research perspectives
Immune escape-associated and drug-resistance mutations.