Published online Jul 7, 2020. doi: 10.3748/wjg.v26.i25.3586
Peer-review started: February 3, 2020
First decision: March 24, 2020
Revised: March 26, 2020
Accepted: May 26, 2020
Article in press: May 26, 2020
Published online: July 7, 2020
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest and most aggressive solid tumors and has a poor prognosis. Transducin (β)-like 1 X-linked receptor 1 (TBL1XR1) has been linked to the progression of various human cancers. Nevertheless, the function and role of TBL1XR1 in pancreatic cancers are unclear.
PDAC is one of the deadliest solid tumors. Identification of diagnostic and therapeutic biomarker of PDAC is urgently needed. TBL1XR1 has been linked to the progression of various human cancers. Nevertheless, the function and role of TBL1XR1 are unclear in pancreatic cancers.
To explore the potential effect and mechanism of TBL1XR1 in the development of PDAC.
Ninety histologically confirmed PDAC patients were enrolled in this study. PDAC cancer samples and cell lines were used to determine the expression of TBL1XR1. CCK-8 assays and colony formation analysis were performed to assess the viability of PDAC cells. Flow cytometry was carried out to analyze changes in the cell cycle and apoptosis. Related protein expression changes were determined by western blot analysis. Animal analysis was performed to confirm the impact of TBL1XR1 in vivo.
The results showed that patients with TBL1XR1-positive tumors had worse overall survival than those with TBL1XR1-negative tumors. Moreover, we found that TBL1XR1 obviously promoted PDAC cell proliferation and inhibited PDAC cell apoptosis. Moreover, knockdown of TBL1XR1 induced G0/G1 phase arrest. In vivo animal studies confirmed that TBL1XR1 accelerated tumor cell growth. The results of western blot analysis showed that TBL1XR1 might play a significant role in regulating PDAC cell proliferation and apoptosis via the PI3K/AKT pathway.
TBL1XR1 promotes PDAC cell progression and might be a probable effective diagnostic and therapeutic marker for pancreatic cancer.
TBL1XR1 may be an effective diagnosis and therapeutic marker for pancreatic cancer