Published online Dec 7, 2019. doi: 10.3748/wjg.v25.i45.6653
Peer-review started: September 17, 2019
First decision: November 4, 2019
Revised: November 8, 2019
Accepted: November 16, 2019
Article in press: November 16, 2019
Published online: December 7, 2019
Acute pancreatitis (AP) is often associated with intestinal injury, which in turn exaggerates the progression of AP. Our recent study has shown that a low level of serum irisin, a novel exercise-induced hormone, is associated with poor outcomes in patients with AP, and irisin administration protects against experimental AP. However, the role of irisin in intestinal injury in AP has not been evaluated.
The main topic of this study was to investigate the effect of irisin administration on intestinal injury in experimental AP. We found that irisin alleviates intestinal cell injury in AP mice by inhibiting oxidative stress and blocking ER stress.
To investigate the effect of irisin administration on intestinal injury in experimental AP. Exogenous irisin can effectively reduce intestinal injury and systemic inflammation in mice with AP. In the future, irisin-related drugs can be developed for intestinal injury of AP.
Arginine-AP was induced by two hourly intraperitoneal injections of 4.0 g/kg L-arginine. At 2 h after the last injection of L-arginine, normal saline (vehicle) or 50 μg/kg or 250 μg/kg irisin was administered through intraperitoneal injection. The animals were sacrificed 69 h after irisin treatment (i.e. 72 h after the first injection of L-arginine). Blood and tissue samples were collected. Intestinal injury, apoptosis, oxidative stress, endoplasmic reticulum stress levels as well as systemic inflammation were measured.
Administration of irisin significantly mitigated intestinal damage, reduced apoptosis, and attenuated oxidative and ER stress in AP mice. In addition, irisin treatment also effectively downregulated serum TNF-α and IL-6 levels and alleviated injury in the pancreas, liver and lung of AP mice. Until now, the exact molecular mechanism by which irisin alleviates intestinal injury in AP remains unknown.
In this study we report for the first time that irisin-mediated multiple physiological events attenuate intestinal injury following an episode of AP. Irisin has a great potential to be further developed as an effective treatment for patients with AP. In summary, irisin alleviates intestinal cell injury in AP mice by inhibiting oxidative stress and blocking ER stress. We believe that future studies can further explore the specific mechanism of irisin relieving intestinal injury in AP. The present study represents an important step forward in the study of irisin in the treatment of AP-associated intestinal injury.
In this study, we can learn that the protective effect of exercise on multiple organs can be revealed by modern science. In the future, our research direction should be to further explore the specific mechanism of irisin’s protective effect on intestinal injury of AP and even multiple organs. Future research should start from animal experiments and carry out more clinical studies so that basic science can make contributions to human health as soon as possible.