Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 21, 2019; 25(39): 5973-5990
Published online Oct 21, 2019. doi: 10.3748/wjg.v25.i39.5973
Differentially expressed long noncoding RNAs and regulatory mechanism of LINC02407 in human gastric adenocarcinoma
Li-Li Zhou, Yan Jiao, Hong-Mei Chen, Li-Hua Kang, Qi Yang, Jing Li, Meng Guan, Ge Zhu, Fei-Qi Liu, Shuang Wang, Xue Bai, Yan-Qiu Song
Li-Li Zhou, Hong-Mei Chen, Li-Hua Kang, Meng Guan, Ge Zhu, Fei-Qi Liu, Shuang Wang, Xue Bai, Yan-Qiu Song, Cancer Center, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Yan Jiao, Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Qi Yang, Jing Li, Department of Radiology, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Author contributions: Zhou LL, Jiao Y, and Chen HM designed this research and drafted the manuscript; Kang LH, Liu FQ, Wang S, and Bai X generated, collected, and analyzed the data; Yang Q and Li J interpreted the data; Guan M and Zhu G critically revised the manuscript; Song YQ conceived the study and revised the manuscript; all authors agree to be fully accountable for ensuring the integrity and accuracy of the work, and read and approved the final manuscript.
Supported by the Science and Technology Department of Jilin Province, No. 20160101028JC; and the Special Funds of Provincial Strategic Adjustment of Economic Structure to Guide in Jilin Province, No. 2014G074.
Institutional review board statement: The study was reviewed and approved by the Ethics Committee of the First Hospital of Jilin University.
Conflict-of-interest statement: The authors declare that there is no conflict of interest related to this study.
Data sharing statement: The datasets supporting the conclusions of this article are included within the article.
ARRIVE guidelines statement: We have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Yan-Qiu Song, MD, PhD, Academic Research, Director, Doctor, Full Professor, Professor, Teacher, Cancer Center, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China. song_yq@jlu.edu.cn
Telephone: +86-431-88783829 Fax: +86-431-88786134
Received: May 27, 2019
Peer-review started: May 27, 2019
First decision: July 21, 2019
Revised: September 4, 2019
Accepted: September 13, 2019
Article in press: September 13, 2019
Published online: October 21, 2019
ARTICLE HIGHLIGHTS
Research background

The study of the role and mechanism of long non-coding RNAs (lncRNAs) in tumor progression has been gradually developed and validated. The expression of lncRNAs in gastric cancer (GC) may be related to the progression of GC. However, there is little experimental evidence about their mechanism of action in GC.

Research motivation

The study of lncRNA-related genes may suggest its carcinogenic effects and potential molecular mechanisms in GC, and may further provide a new direction for the diagnosis and treatment of GC.

Research objectives

The main objectives of our study were to investigate the differential expression of lncRNAs in human GC and elucidate the function and regulatory mechanism of LINC02407.

Research methods

Quantitative real-time PCR was used to detect lncRNA gene expression in GC tissues and matched adjacent non-tumor tissues and used the Cancer Genome Atlas database to verify the role of lncRNAs in GC. In a further molecular mechanism study, we confirmed the possible molecular mechanisms and regulatory pathways by which LINC02407 exerts its role by overexpressing and knocking down the expression of downstream molecules of LINC02407.

Research results

LncRNA LINC02407 was up-regulated in GC tissues and cell line and promoted proliferation and metastasis and inhibited apoptosis of GC cells. LINC02407 played a role in GC through the LINC02407-miR-6845-5p/miR-4455-adhesion G protein-coupled receptor D1 (ADGRD1) pathways, and thus, it may be an important oncogene and has potential value in GC diagnosis and treatment.

Research conclusions

The authors demonstrated that LINC02407 is overexpressed in GC tissues and cell lines, which could provide more evidence for the clinical use of lncRNAs as biomarkers in GC. We also confirmed that LINC02407 plays a role in GC through the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways. Overexpression of LINC02407 may reflect a promising treatment strategy for GC, which calls for more validated data in the future.

Research perspectives

Through this study, we will have a deeper understanding of the role and mechanism of lncRNAs in GC in the future. And members of the LINC02407-miR-6845-5p/miR-4455-ADGRD1 axis can be useful targets for future prevention and treatment innovations in GC.