Published online Jun 21, 2019. doi: 10.3748/wjg.v25.i23.2924
Peer-review started: November 22, 2018
First decision: December 12, 2018
Revised: March 12, 2019
Accepted: March 29, 2019
Article in press: March 30, 2019
Published online: June 21, 2019
Severe infection is one of the most common causes of gastrointestinal dysfunction, and its pathogenesis is closely related to endotoxemia and intestinal barrier injury.
Bifidobacterium plays an important role in maintaining the integrity of the intestinal mucosal barrier.
This study investigated the protective mechanism of Bifidobacterium during ileal injury in rats.
Using endotoxin injured rat models, ileal cytokine-induced neutrophil chemoattractant (CINC) mRNA expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR), and expression of ileal insulin-like growth factor 1 (IGF-1) protein and mRNA was detected by immunohistochemistry and RT-PCR, respectively.
There was a significant difference in CINC mRNA expression between the different groups (P < 0.05). There was a significant difference in IGF-1 brown granule expression among the different groups (P < 0.05), and expression of IGF-1 mRNA significantly differed among the three groups (P < 0.05)
Bifidobacterium may increase IGF-1 expression and enhance intestinal immune barrier function in rats with endotoxin injury.
This study can provide a new therapeutic tool and theoretical support for gastrointestinal dys-function.