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©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2003; 9(9): 2078-2082
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.2078
Protein kinase C/ζ (PRKCZ) Gene is associated with type 2 diabetes in Han population of North China and analysis of its haplotypes
Yun-Feng Li, Hong-Xia Sun, Guo-Dong Wu, Wei-Nan Du, Jin Zuo, Yan Shen, Bo-Qin Qiang, Zhi-Jian Yao, Heng Wang, Wei Huang, Zhu Chen, Mo-Miao Xiong, Yan Meng, Fu-De Fang
Yun-Feng Li, Hong-Xia Sun, Guo-Dong Wu, Wei-Nan Du, Jin Zuo, Yan Meng, Fu-De Fang, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
Yan Shen, Bo-Qin Qiang, Zhi-Jian Yao, Chinese National Human Genome Center at Beijing, Beijing, 100176, China
Wei Huang, Zhu Chen, Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China
Heng Wang, Peking Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China
Mo-Miao Xiong, Human Genetics Center, Health Science Center, The University of Texas, Houston TX 77225, USA
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39896200, No30170441, the National High Technology Research and Development Program, No. 2001AA221161, No. 2002BA711A05, No. 2002BA711A10-02. The National Program for Key Basic Research Projects, No. G1998051016, the Natural Science Foundation of Beijing, No. 7002026
Correspondence to: Fu-De Fang and Yan Meng, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. fangfd@public3.bta.net.cn, ymengsmile@yahoo.com
Telephone: +86-10-65253005 Fax: +86-10-65253005
Received: January 18, 2003
Revised: January 29, 2003
Accepted: March 3, 2003
Published online: September 15, 2003
AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.
METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Han ethical origin, to perform this case-control study. The haplotypes with significant difference in the gene (s) were further analyzed.
RESULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP, rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P < 0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.
CONCLUSION: PRKCZ gene may be associated with type 2 diabetes in Han population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.
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