Jiang YA, Luo HS, Zhang YY, Fan LF, Jiang CQ, Chen WJ. Telomerase activity and cell apoptosis in colon cancer cell by human telomerase reverse transcriptase gene antisense oligodeoxynucleotide. World J Gastroenterol 2003; 9(9): 1981-1984 [PMID: 12970889 DOI: 10.3748/wjg.v9.i9.1981]
Corresponding Author of This Article
Ying-An Jiang, Central Hospital of Huangshi City, 43 Wuhan Road, Huangshi 435000, Hubei Province China. weijin@hs.hb.cninfo.net
Article-Type of This Article
Colorectal Cancer
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Ying-An Jiang, He-Sheng Luo, Department of Gastroenterology, Renming Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Li-Fang Fan, Department of Pathology, Medical College of Wuhan University, Wuhan 430071, Hubei Province, China
Chong-Qing Jiang, Department of General surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China
You-Yuan Zhang, Department of Pathology, Central Hospital of Huangshi City, Huangshi 435000, Hubei Province, China
Wei-Jin Chen, Department of Medical information, Central Hospital of Huangshi City, Huangshi 435000, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Science and Technology Research Project of Hubei Province, No. 2002AA301C72
Correspondence to: Ying-An Jiang, Central Hospital of Huangshi City, 43 Wuhan Road, Huangshi 435000, Hubei Province China. weijin@hs.hb.cninfo.net
Telephone: +86-714-6283783 Fax: +86-714-6233931
Received: March 4, 2003 Revised: April 6, 2003 Accepted: April 19, 2003 Published online: September 15, 2003
Abstract
AIM: To evaluate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (As-ODN) on telomerase activity and cell apoptosis in colon cancer cell line SW480.
METHODS: As-ODN was transfected into cells SW480 by liposomal transfection. Cultured cells were divided into three groups: ASODN (5’GGAGCGCGCGGCATCGCGGG-3’), sense oligodeoxynucleotide (5’-CCCGCGATGCCGCGCGCTCC-3’, S-ODN) and control. The concentration of oligodeoxynucleotide and lipsome was 10 μmol/L and 16 mg/L, respectively. The activity of telomerase was examined by telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (ELISA), and cell apoptosis was observed by morphology and flow cytometry in each group.
RESULTS: Telomerase activity began to be down-regulated or inhibited when cells SW480 were treated with As-ODN for 72 h, and cell apoptosis was induced.
CONCLUSION: It is suggested that hTERT As-ODN might specially inhibit the activity of telomerase in colon cancer cells and it is further proved that the hTERT gene has a significant correlation with telomerase activity. Further evidence is needed to prove whether hTERT As-ODN is a potential tool for the treatment of colon cancer.
