Esophageal Cancer
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2002; 8(5): 777-781
Published online Oct 15, 2002. doi: 10.3748/wjg.v8.i5.777
Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry
Xing-Dong Xiong, Li-Yan Xu, Zhong-Ying Shen, Wei-Jia Cai, Jian-Min Luo, Ya-Li Han, En-Min Li
Xing-Dong Xiong, Jian-Min Luo, En-Min Li, Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515031, Guangdong Province, China
Li-Yan Xu, Zhong-Ying Shen, Wei-Jia Cai, Department of Pathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China
Ya-Li Han, Department of Biology, Shantou University, Shantou 515031, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China, NO.39900069, NO.30170428; Guangdong Provincial Natural Science Foundation, NO.990799, NO.010431; Guangdong provincial College Natural Science Foundation, NO.200033; Guangdong provincial medical Scientific Foundation, NO.A2001419 and Research and Development Foundation of Shantou University, NO.L0004, NO.L00012.
Correspondence to: Dr. En-Min Li, Department of Biochemistry and Molecular Biology, Shantou University Medical College, 22 Xinling Road, Shantou 515031, Guangdong Province, China. nmli@21cn.com
Telephone: +86-754-8532720
Received: May 18, 2002
Revised: June 22, 2002
Accepted: June 27, 2002
Published online: October 15, 2002
Abstract

AIM: To identify the differentially expressed proteins between the human immortalized esophageal epithelial cell line (SHEE) and the malignant transformed esophageal carcinoma cell line (SHEEC), and to explore new ways for studying esophageal carcinoma associated genes.

METHODS: SHEE and SHEEC cell lines were used to separate differentially expressed proteins by two-dimensional electrophoresis. The silver-stained 2-D gels was scanned with EDAS290 digital camera system and analyzed with the PDQuest 6.2 Software. Six spots in which the differentially expressed protein was more obvious were selected and analyzed with matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).

RESULTS: There were 107±4.58 and 115±9.91 protein spots observed in SHEE and SHEEC respectively, and the majority of these spots between the two cell lines matched each other (r = 0.772), only a few were expressed differentially. After analyzed by MALDI-TOF-MS and database search for the six differentially expressed proteins, One new protein as well as other five sequence-known proteins including RNPEP-like protein, human rRNA gene upstream sequence binding transcription factor, uracil DNA glycosylase, Annexin A2 and p300/CBP-associated factor were preliminarily identified.

CONCLUSION: These differentially expressed proteins might play an importance role during malignant transformation of SHEEC from SHEE. The identification of these proteins may serve as a new way for studying esophageal carcinoma associated genes.

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