Original Research
Copyright ©The Author(s) 2001. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2001; 7(5): 678-684
Published online Oct 15, 2001. doi: 10.3748/wjg.v7.i5.678
Pathogenic and pathological characteristic of new type gosling viral enteritis first observed in China
An-Chun Cheng, Ming-Shu Wang, Xiao-Yue Chen, Yu-Fei Guo, Zhao-Yu Liu, Peng-Fei Fang
An-Chun Cheng, Ming-Shu Wang, Xiao-Yue Chen, Yu-Fei Guo, Zhao-Yu Liu, Peng-Fei Fang, College of Animal Science and Veterinary Medicine, Sichuan Agricultural University, Xinkang Road, Ya’an City 625014, Sichuan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Nature Scientific Foundation of China, No.39970561 and the Funds for the Leaders in Academia of Sichuan Province, No.9900009240018
Correspondence to: An-Chun Cheng, College of Animal Science and Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, Sichuan Province, China. Chenganchun@Hotmail.com; Anchun@sicau.edu.cn
Telephone: +86-835-2226327/2242544/2242326, Fax: +86-835-2242754
Received: June 12, 2001
Revised: July 26, 2001
Accepted: August 2, 2001
Published online: October 15, 2001

AIM: To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV), the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological development in goslings infected artificially with NGVEV.

METHODS: ① NGVEV virions were purified by the procedure of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration chromatography, and SDS-PAGE. ② Forty 2-day-old White Sichuan goslings were orally administered with NGVEV and 24 h later 2 birds were randomly selected and killed at 24 h intervals until death occurred. Specimens (duodenum, ileum, liver, heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and eosin and studied by light microscope.

RESULTS: NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15 polypeptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodenum were first observed in goslings 2 d postinfection artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of mucosa necrosis and inflammatory exudation of the small intestine, fibrinonecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudomembrane or of the mixture of fibrous exudate and necrotic intestinal mucosa were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on the lung and kidney. Epithelial cells of the renal tubular underwent degeneration. In some cases, granular degeneration and fatty degeneration could be found in the liver and in some cases at a later stage of this disease the epithelial cells of trachea and proventriculus might be found sloughed. In some cases at an early stage of this disease, cardiac hyperemia and hemorrhage could be observed. Esophagus, pancreas and brain were found normal. Analyses and comparisons between the pathologic lesions of NGVE and Gosling Plague (GP) were available in this paper as well.

CONCLUSION: ① NGVEV is adenovirus. ② Pathological characteristic could be as the data for NGVE diagnosis.

Keywords: enteritis/virology, enteritis/pathology, adenoviridae/isolation & purification, gosling/virology, gosling/new infectious disease