Abstracts
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 84-84
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.84
Effect of boschniakia rossica on expression of GST-P, p53 and p21ras proteins in early-stage chemical hepatocarcinogenesis and its anti-inflammatory activities in rats
Zong-Zhu Yin, Hai-Ling Jin, Xue-Zhe Yin, Tian-Zhu Li, Ji-Shu Quan, Zeng-Nan Jin
Zong-Zhu Yin, Hai-Ling Jin, Xue-Zhe Yin, Tian-Zhu Li, Ji-Shu Quan, Zeng-Nan Jin, Institute for Cancer Research, Yanbian University College of Medicine, Yanji 133000, Jilin Province, China
Author contributions: All authors contributed equally to the work.
Supported by The National Natural Science Foundation of China, No. 39660021
Correspondence to: Dr. Zong-Zhu Yin, Professor, Institute for Cancer Research, Yanbian University College of Medicine, 121 Juzi Street, Yanji 133000, Jilin Province, China. yinzz@public.yj.jl.cn
Telephone: 433-2660589 Fax: 433-2621142
Received: April 24, 2000
Revised: May 20, 2000
Accepted: June 10, 2000
Published online: September 15, 2000
Abstract

AIM: To investigate the effect of boschniakia rossica (BR) extract on expression of GST-P, p53 and p21ras proteins in early stage chemical hepatocarcinogenesis in rats and its anti-inflammatory actions.

METHODS: The expression of tumor marker, placental form glut athione S-transferase (GST-P), p53 and p21ras proteins were investigated by immunohistochemical techniques and ABC method. Anti-inflammatory activities of BR were observed by xylene and croton oil-induced mouse ear edema, carrageenin, histamine and hot scald-induced rat pow edema, adjuvant-induced rat arthritis and cotton pellet induced mouse granuloma formation methods.

RESULTS: The 500 mg/kg of BR-H2O extract fractionated from BR Methanol extract had inhibitory effect on the formation of DEN-induced GST-P-positive foci in rat liver and the expression of mutant p53 and p21ras protein was lower than that of hepatic preneoplastic lesions. Both CH2Cl2 and H2O extract from BR have inhibitory effect in xylene and croton oil-induced mouse ear edema. BR-H2O extract exhibited inhibitory effect in carrageenin, histamine and hot scald-induced hind paw edema and adjuvant-induced arthritis in rats and cotton pellet induced granuloma formation in mice.

CONCLUSION: BR extract exhibited inhibitory effect on formation of Preneoplastic hepatic foci in early stage of rat chemical hepatocarcinogenesis. Both CH2Cl2 and H2O extract from BR exerted anti-inflammatory effect in rats and mice.

Keywords: Boschniakia rossica; Liver neoplasms/chemically induced; Glutathione transferases; Protein p53; Proto-oncogene protein p21 (Ras); Immunohistochemistry; Arthritis, adjuvant; Antibiotics