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©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 69-69
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.69
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.69
Point mutations in E2, NS3 and NS5A of hepatitis G virus
Wei-Dong Liu, Department of Infectious Diseases, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China
Toshikazu Hada, Ji-Dong Cheng, Kazuya Higashino, Third Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Wei-Dong Liu, Department of Infectious Diseases, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China. wdliu@mailserv.stu.edu.cn
Telephone: 754-8341233-3114 Fax: 754-8346543
Received: February 22, 2000
Revised: March 20, 2000
Accepted: May 10, 2000
Published online: September 15, 2000
Revised: March 20, 2000
Accepted: May 10, 2000
Published online: September 15, 2000
Abstract
AIM: To compare the point mutation deviations of HGV among E2, NS3 and NS5A.
METHODS: Seven patients with hepatic diseases from Japan and China were selected for this study. RNA was extracted and amplified by semi-nested RT-PCR; and the PCR products were sequenced directly.
RESULTS: The point mutation deviations of HGV in E2, NS3 and NS5A were 10%-17%, 11%-23%, and 0%-5%, in nucleotide sequences and 4%-12%, 0%, and 0%-6% in amino acid sequences respectively.
CONCLUSION: The frequency of variation at the nucleotide level was in the order NS3 > E2 > NS5A, while at the amino acid level the order was E2 > NS5A > NS3. The detected sequences from the N-terminus of E2 may be the poorly conserved region of HGV.
Keywords: Hepatitis G virus, Genes, Mutation, Base sequence, Amino acid sequence