Abstracts
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 49-49
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.49
Effects of nitric oxide synthesis inhibitor in long-term treatment on hyperdynamic circulatory state in cirrhotic rats
Ying-Qiu Huang, Shu-Dong Xiao, Jian-Zhong Mo, De-Zhong Zhang
Ying-Qiu Huang, Department of Gastroenterology, General Hospital of Benxi Iron and Steel Co, Benxi 117000, Liaoning Province, China
Shu-Dong Xiao, Jian-Zhong Mo, De-Zhong Zhang, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Ying-Qiu Huang, Department of Gastroenterology, General Hospital of Benxi Iron and Steel Co., Benxi 117000, Liaoning Province, China
Telephone: 414-2845501-473 Fax: 414-7828421
Received: January 10, 2000
Revised: June 5, 2000
Accepted: July 10, 2000
Published online: September 15, 2000
Abstract

AIM: To investigate the effects of low dosage of nitric oxide synthesis (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) in long-term treatment on hyperdynamic circulatory state in rats with cirrhosis.

METHODS: Cirrhosis model was induced in male SD rats by injection of 60% CCl4 oily solution subcutaneously. Cirrhotic rats were treated with L-NAME (0.5 mg·kg-1·d-1) by gavage for two weeks. Mean arterial pressure (MAP), cardiac output (CO), cardiac index (CI), splanchnic vascular resistance (SVR), splanchnic blood flow (SBF) and serum NO levels were determinded in L-NAME-treated, L-NAME-untreated cirrhotic rats and controls by using 57 Co Labled microsphere technique and a fluorometric assay, respectively.

RESULTS: Untreated cirrhotic rats had significantly lower MAP, SVR and higher PP, CO, CI, SBF and NO concentration than controls (14.42 ± 0.47 kPa vs 17.05 ± 0.34 kPa, 2.974 ± 0.186 kPa·mL-1·min-1vs 4.234 ± 0.118 kPa·mL-1·min-1, 1.665 ± 0.067 kPa vs 1.123 ± 0.096 kPa, 189.99 ± 9.26 mL/min vs 135.5 ± 3.55 mL/min, 55.89 ± 1.82 mL-1·mL-1·100 g-1 BW vs 39.68 ± 1.64 mL-1·mL-1·100 g-1 BW, 4.60 ± 1.25 μmol/L vs 0.53 ± 0.26 μmol/L, P < 0.01, respectively=. In treated cirrhotic rats, L-NAME significantly attenuated the increase of CO, CI, SBF, NO concentration and the decrease of MAP and SVR. In treated cirrhotic rats, L-NAME induced a marked decrement of NO concentration than untreated cirrhotic rats (1.471 ± 0.907 μmol/L vs 4.204 ± 1.253 μmol/L, P < 0.01).

CONCLUSION: The endogenous NO may play an important role in the changes of hemodynamics pattern in cirrhosis, and hyperdynamic circulatory state in rats with cirrhosis can be ameliorated by long term low dose L-NAME treatment.

Keywords: Nitric oxide synthase; Hemodynamics; Fibrosis; Microsphere; Fluorometry; Rats