Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.116
Revised: June 27, 2000
Accepted: July 10, 2000
Published online: September 15, 2000
AIM: To establish a model system for studying gastric carcinogenesis of MNNG, a gastric cancer related carcinogen.
METHODS: Cell culture transformation, PCR restriction fragment length polymorphism (PCR-RFLP), DNA blotting and immunochemical techniques and analysis of LDH isozyme and chromosome were performed.
RESULTS: GES-1 cells surviving by MNNG treatment were named MC (2 × 105 M for 24 h) and MC B (2 × 107 M for 7 d). The two cell lines treated by MNNG showed more malignant than maternal cell GES-1 with the evidences of more chromosome aberrations, abnormal morphology and cytoskeleton and also gained the ability of colony formation on soft agar. C-Ha-ras gene point mutation in the 12th codon and LDH isoenzyme abnormal express were found in MC-B cells. In addition, C-met gene rearrangement was revealed by Southern blot analysis in MC-B and MC.
CONCLUSION: This gastric epithelial cell system is an important model system for further study of stomach cancer, MNNG had a selective effect on the cytoskeleton microfilament in human gastric epithelial cells and intimately associated with the activation of certain oncogenes and some protein.