Original Articles
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2000; 6(2): 216-219
Published online Apr 15, 2000. doi: 10.3748/wjg.v6.i2.216
Inhibitory effects of Curcuma aromatica oil on proliferation of hepatoma in mice
Wan Yin Wu, Qin Xu, Ling Chun Shi, Wei Bin Zhang
Wan Yin Wu, Department of Cancer, the Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120, Guangdong Province, China
Ling Chun Shi, Wei Bin Zhang, Department of Pathology, the Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120, Guangdong Province, China;
Qin Xu, Department of Pathology, Guangzhou University of TCM, Guangzhou 510405, Guangdong Province, China;
Wan Yin Wu, male, born on 1964-08-10 in He County, Anhui Province, graduated from Anhui TCM College as bachelor in 1986, Shanghai TCM University as M.D. in 1992, and Shanghai Medical University as Ph.D. in 1997. Now he is a postdoctoral fellow, associate professor, specializing in prevention and treatment of hepatic neoplasms with integrated Chinese and western medicine, having 28 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Wan Yin Wu, Department of Cancer, the Second Affiliated Hospital of Guangzhou University of TCM, 111 Dadelu, Guangzhou 510120, Guangdong Province, China. wwanyin@hotmail.com
Telephone: 0086-20-81887233 Ext.368
Received: June 23, 1999
Revised: December 6, 1999
Accepted: December 25, 1999
Published online: April 15, 2000
Abstract

AIM: To reveal the inhibitory effects of Curcuma aromatica oil (CAO) on cell proliferation of hepatoma in mice.

METHODS: Two tumor inhibitory experiments of CAO on hepatoma in mice were conducted. The inhibitory effects of CAO on proliferation of hepatoma in mice were evaluated by DNA image cytometry and immunohistochemical staining of proliferating cell nuclear antigen (PCNA).

RESULTS: The tumor inhibitory rates of CAO were 52% and 51% in two experiments, respectively. Compared with those of the saline-treated control groups, both differences were statistically significant (P ＜ 0.01). In the group of mice treated with CAO, the cellular nuclear DNA OD value (249 ± 70), are as (623 μm2± 228 μm2) and DNA (2.38 ± 0.67) index of hepatic carcinomas were significantly lower than those of the control group (430 ± 160, 1073 μm2± 101 μm2 and 4.48 ± 0.71). CAO also could increase diploidy cell rates (29.00% ± 9.34% vs 2.97% ± 5.69%, P ＜ 0.01) and decrease pentaploidy cell exceeding rate (30.04% ± 15.10% vs 70.89% ± 14.94%, P ＜ 0.01). In the group of mice treated with CAO, the labeling indexes of proliferating cell nuclear antigen (PCNA-LI) were 30% ± 4%, which were significantly lower than 40% ± 6% of the control group (P ＜ 0.01).

CONCLUSION: The inhibition of CAO on the growth of hepatoma in mice might be associated with its depression on cellular proliferative activity.

Keywords: Curcuma aromatica-oil, liver neoplasms, cell proliferation, mice