Published online Oct 15, 1998. doi: 10.3748/wjg.v4.iSuppl2.126
Revised: August 11, 1998
Accepted: September 9, 1998
Published online: October 15, 1998
AIM: We investigated the role of infection by HCMV AD169 strain in gastroenteropathy.
METHODS: Forty rats were divided into three groups at random-t wenty of viral inoculation, ten of inactive virus control, ten of normal control. HCMV AD169 strain was injected into caudal venous as 104TCID50 A nimals were killed and dissected in 50 d. Their gastric, enteral and hepatic tissues were observed by pathological methods and HCMV antigen was detected by immunohistochemistry in situ. Virus extracted from liver were recovered in HEL, then pathergic cell was manifested by electron microscope and its supernate was determined by ELISA for HCMV antigen.
RESULTS: The tissues from two groups of control animals were normal. Seven of viral inoculated animals died and pathological alternatives were observed that focal gastroenteric mucous membrane degenerated and necrosed, even deeper into smooth muscle layer and lymphocytes infiltrated around the focus, hepatomegaly or hepatatrophy with hyperemia, hemorrhage and necrosis, compensational hyperplasia emerged round the focus. HCMV located in gastroenteric mucous membrane cell. hepatocyte, cholangiolar epithelial cell. Virus extracted from rats died of inoculation was recovered in HEL. Cytopathic effects appeared in 12 d, groups of viral particulates were found in nucleus of pathergic hepatocyte by electron microscope and HCMV antigen was determined from its supernate by ELISA.
CONCLUSION: HCMV can infect gastroenteric mucous membrane cell and hepatocyte with serious pathological damage exhibited as degeneration necro sis and lymphocyte infiltration.