Published online Dec 15, 1997. doi: 10.3748/wjg.v3.i4.251
Revised: May 2, 1997
Accepted: October 28, 1997
Published online: December 15, 1997
AIM: To study the relationship between β-glucuronidase and the invasiveness of human colorectal carcinoma cell lines.
METHODS: Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman’s method to determine the concentration of β-glucuronidase in the medium.
RESULTS: Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229).
CONCLUSION: Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.